Japan, Feb. 27 -- ZHEJIANG UNIV has got intellectual property rights for 'INFECTIOUS PLANT RHABDOVIRUS VECTOR, AND METHOD FOR SITE-SPECIFICALLY EDITING NON-TRANSGENIC GENOME.' Other related details are as follows:
Application Number: JP,2024-030532
Category (FI): A01H1/00@A,A01H6/82,C12N15/09,100,C12N15/09,110,C12N15/47,C12N15/83@Z,C12N5/04,C12N5/10,C12N7/01
Stage: PROBLEM TO BE SOLVED: To provide methods for site-specifically modifying a plant chromosomal DNA using a plant rhabdovirus vector, and to provide viral vector compositions.SOLUTION: A step of systemically infecting a plant using a plant rhabdovirus vector, expressing a sequence-specific nuclease, targeting an infected plant genomic target site with a sequence-specific nuclease, and cleaving the target site; and a step of completing a site-specific editing on the target sites by a DNA repair mechanism of the plant are comprised. This method for site-specifically editing a plant genome target DNA does not require the introduction of selective marker genes. By delivering a sequence-specific nuclease using a plant rhabdovirus expression vector, not only can the editing efficiency for the target site be improved, but also a non-transgenic, and stably inheritable edited plant can be obtained.SELECTED DRAWING: Figure 1 (Grant)
Filing Date: Feb. 29, 2024
Publication Date: April 16, 2024
The original document can be viewed at: https://www.j-platpat.inpit.go.jp/p0100
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