Japan, Feb. 27 -- TOYOBO CO LTD,INSTITUTE OF PHYSICAL & CHEMICAL RESEARCH has got intellectual property rights for 'METHOD FOR SUPPRESSING NON-SPECIFIC NUCLEIC ACID AMPLIFICATION.' Other related details are as follows:
Application Number: JP,2019-176436
Category (FI): C12Q1/6876@Z,C12N15/09@Z,C12Q1/6844@Z,C12N9/22,C12N9/12
Stage: PROBLEM TO BE SOLVED: To provide a method for suppressing non-specific nucleic acid amplification in an RT-RamDA reaction.SOLUTION: The present invention provides a method for suppressing non-specific nucleic acid amplification that may occur in an RT-RamDA reaction solution by coexisting a template RNA and a chaotropic agent in the RT-RamDA reaction solution. Preferably, the final concentration of the chaotropic agent in the RT-RamDA reaction solution is more than 0 mM and 50 mM or less. The chaotropic agent is preferably at least one selected from the group consisting of guanidinium ion, urea ion, iodide ion, lithium ion, and salts thereof.SELECTED DRAWING: None (Grant)
Filing Date: Sept. 27, 2019
Publication Date: April 8, 2021
The original document can be viewed at: https://www.j-platpat.inpit.go.jp/p0100
Disclaimer: Curated by HT Syndication.
